stationary phase role
IRI: https://spec.industrialontologies.org/ontology/construct/StationaryPhaseRole
Defined In: https://spec.industrialontologies.org/ontology/biopharma/BiopharmaEquipment/
SubClass Of: bfo:role
Class Hierarchy
owl:Thing › bfo:entity › bfo:continuant › bfo:specifically dependent continuant › bfo:realizable entity › bfo:role › stationary phase role
Definition
role held by a solid, gel, or liquid that is planned to be or used in a chromatography process as the immobilized material which selectively and reversibly retains solutes in the mobile phase
Explanatory Notes
1) Common terms such as “chromatographic bed” or “sorbent” may refer to its various physical forms. To avoid ambiguity, expressions like “liquid stationary phase” or “solid stationary phase” are preferred when specifying its physical state 2) The stationary phase selectively and reversibly retains solutes, enabling their separation during chromatography through differential interactions with both the stationary and mobile phases. This includes mechanisms such as size exclusion, ion exchange, and affinity binding.
AFO_/role#AFRL:0000032 and https://goldbook.iupac.org/terms/view/S05949 and “https://userpages.umbc.edu/~dfrey1/documents/lc_glossary.pd
Examples
- Silica gel as stationary phase in normal-phase chromatography; C18 bonded silica as stationary phase in reverse-phase chromatography; agarose bead gel as stationary phase in size-exclusion chromatography; ion-exchange resin as stationary phase in ion-exchange chromatography; protein A immobilized on resin as stationary phase in affinity chromatography; polymeric resin as stationary phase in hydrophobic interaction chromatography.
Primitive Class
This class is declared primitive and it does not have necessary and sufficient condiftions defined.
Primitive Rationale
There are insufficient constructs to create necessary and sufficient conditions. In particular modeling of solid,gel or liquid is required.
Formal Axioms
First-Order Logic Axioms
StationaryPhaseRole(x) → Role(x) ∧ ∃y(MaterialEntity(y) ∧ roleOf(x, y))
Semi-Formal Natural Language Axioms
if x is a ‘stationary phase role’ then x is a ‘role’ and x is the ‘role of’ some ‘material entity’
Description Logic
constr:StationaryPhaseRole ⊑ bfo:role
constr:StationaryPhaseRole ⊑ ∃ constr:roleOf .bfo:material_entity
SubClassOf: bfo:role
SubClassOf: constr:roleOf some bfo:material_entity
constr:StationaryPhaseRole DisjointWith: constr:MobilePhaseRole