cloned cell line
IRI: https://spec.industrialontologies.org/ontology/construct/ClonedCellLine
Defined In: https://spec.industrialontologies.org/ontology/biopharma/Material/
SubClass Of: cell line
Class Hierarchy
owl:Thing › bfo:entity › bfo:continuant › bfo:independent continuant › bfo:material entity › bfo:object aggregate › cell population › cultured cell population › cell line › cloned cell line
Definition
cell line that originates from a single ancestral cell
Explanatory Notes
1) A cloned cell line consists of cells that are genetically identical with respect to antigen recognition, gene sequence, and key phenotypic traits. It represents a uniform lineage of cells with shared ancestry and consistent functional characteristics. 2) Regulatory standards in biologics require proof of monoclonality for a production cell line. This implies that it originates from a single progenitor cell, regardless of whether one calls it a clone or a line. In practice, a clone is the starting point for development: once selected and scaled, it becomes a permanent cell line with specific attributes used for product manufacture. 3) FDA considers monoclonality essential: production cell lines must originate from a single progenitor cell to ensure consistent and safe product characteristics. Each clone selected after transfection or genetic manipulation is treated as a distinct cell line, subject to full characterization (identity, purity, adventitious agent testing, etc.)
Examples
- CHO DG44 clone 5F7 – a clonal derivative of CHO DG44 cells selected for high monoclonal antibody productivity; Jurkat E6-1 – a cloned subline of Jurkat cells used in T cell signaling research
Adapted From
- https://www.sciencedirect.com/topics/biochemistry-genetics-and-molecular-biology/cell-clone#:~:text=Clone%20of%20cells%20is%20a,ignored%20that%20may%20be%20different and CLO:0000177
Primitive Class
This class is declared primitive and it does not have necessary and sufficient condiftions defined.
Primitive Rationale
There are insufficient constructs to define necessary and sufficient conditions.
Formal Axioms
First-Order Logic Axioms
ClonedCellLine(x) → CellLine(x)
Semi-Formal Natural Language Axioms
if x is a ‘cloned cell line’ then x is a ‘cell line’
Description Logic
SubClassOf: constr:CellLine