harvesting process
IRI: https://spec.industrialontologies.org/ontology/construct/HarvestingProcess
Defined In: https://spec.industrialontologies.org/ontology/biopharma/ManufacturingExecution/
SubClass Of: purification process
Class Hierarchy
owl:Thing › bfo:entity › bfo:occurrent › bfo:process › planned process › manufacturing process › purification process › harvesting process
Definition
purification process that has as input the specified output of a biomanufacturing production process and that results in the separation or recovery of biotechnology products from the associated process streams, typically through the isolation or removal of cells, cell debris, or other bulk impurities
Semi-Formal Definition:
every instance of ‘harvesting process’ is defined exactly as an instance of ‘purification process’ that ‘has input’ some ‘process intermediate material’ which ‘is specified output of’ some ‘biomanufacturing production process’ and that ‘is prescribed by’ some ‘recipe process stage’
Explanatory Notes
Harvesting utilizes one or more sequential unit operations, such as centrifugation, filtration, cell concentration and membrane separation.The specific unit operations depend on whether the target product is located inside the cells (intracellular) or secreted into the medium (extracellular), or the cells themselves.
Examples
- Harvesting was carried out by depth filtration of a CHO cell culture to remove cells and debris, yielding a clarified supernatant containing the secreted monoclonal antibody for downstream purification; Harvesting was performed by collecting E. coli cells at early log phase (OD₆₀₀ ≈ 0.3–0.4) during cultivation at 37 °C. The culture was rapidly chilled on ice and centrifuged at 4 °C and 4,000 × g for 10 minutes to separate the biomass from the culture medium;
Adapted From
- https://books.google.rs/books?hl=sr&lr=&id=p-rKBQAAQBAJ&oi=fnd&pg=PP1&dq=bioseparation+processes+harvesting&ots=eFDtLSUJ0I&sig=XjzTFnAmknXNjFlSWHpkkTtpHg0&redir_esc=y#v=onepage&q=bioseparation%20processes%20harvesting&f=false”
Formal Axioms
First-Order Logic Definition
HarvestingProcess(x) ↔ PurificationProcess(x) ∧ ∃m∃p∃s(ProcessIntermediateMaterial(m) ∧ BiomanufacturingProductionProcess(p) ∧ isSpecifiedOutputOf(m, p) ∧ hasInput(x, m) ∧ RecipeProcessStage(s) ∧ prescribedBy(x, s))
Description Logic
constr:HarvestingProcess ≡ constr:PurificationProcess ⊓ ∃ constr:hasInput .(constr:ProcessIntermediateMaterial ⊓ ∃ constr:isSpecifiedOutputOf .constr:BiomanufacturingProductionProcess) ⊓ ∃ constr:prescribedBy .constr:RecipeProcessStage
EquivalentTo: constr:PurificationProcess and constr:hasInput some (constr:ProcessIntermediateMaterial and constr:isSpecifiedOutputOf some constr:BiomanufacturingProductionProcess) and constr:prescribedBy some constr:RecipeProcessStage
SubClassOf: constr:PurificationProcess