cell culture
IRI: https://spec.industrialontologies.org/ontology/construct/CellCulture
Defined In: https://spec.industrialontologies.org/ontology/biopharma/BiopharmaMaterial/
SubClass Of: bfo:material entity
Class Hierarchy
owl:Thing › bfo:entity › bfo:continuant › bfo:independent continuant › bfo:material entity › cell culture
Definition
material entity that has cultured cells and the media in which they are being propagated or stored as parts
Explanatory Notes
A cell culture includes the cells in culture, as well as the media and all additives in which the cells are being grown or in which they are stored.
Examples
- CHO Suspension Culture in CD-CHO Medium; HEK293 cells growing as an adherent monolayer when cultured in DMEM (Dulbecco’s Modified Eagle’s Medium) + 10% FBS (Fetal Bovine Serum)
Counter-Examples
- In situ cells are observed and studied within their natural tissue context without being cultured or removed, e.g., neurons, alveolar epithelial cells; Post-centrifugation cell pellet; Uninoculated Culture Medium; Glass Slide with Fixed Cells (No Live Media); Tissue Biopsy in PBS Buffer
Synonyms
- culture broth
Adapted From
- OBI:0001876
Primitive Class
This class is declared primitive and it does not have necessary and sufficient condiftions defined.
Primitive Rationale
There are insufficient constructs to create a set of necessary and sufficient conditions.
Formal Axioms
First-Order Logic Axioms
CellCulture(x) → MaterialEntity(x) ∧ ∃y (CultureMedium(y) ∧ hasContinuantPartAtAllTimes(x, y)) ∧ ∃z (CulturedCellPopulation(z) ∧ hasContinuantPartAtAllTimes(x, z))
Semi-Formal Natural Language Axioms
if x is a ‘cell culture’ then x is a ‘material entity’ and x ‘has continuant part at all times’ some ‘culture medium’ and x ‘has continuant part at all times’ some ‘cultured cell population’
Description Logic
constr:CellCulture ⊑ bfo:material_entity
constr:CellCulture ⊑ ∃ bfo:has_continuant_part_at_all_times .constr:CultureMedium
constr:CellCulture ⊑ ∃ bfo:has_continuant_part_at_all_times .constr:CulturedCellPopulation
constr:CellCulture ⊓ constr:Cell ⊑ ⊥
SubClassOf: bfo:material_entity
SubClassOf: bfo:has_continuant_part_at_all_times some constr:CultureMedium
SubClassOf: bfo:has_continuant_part_at_all_times some constr:CulturedCellPopulation
constr:CellCulture DisjointWith: (constr:Cell, constr:CultureMedium, constr:CulturedCellPopulation)