culture medium
IRI: https://spec.industrialontologies.org/ontology/construct/CultureMedium
Defined In: https://spec.industrialontologies.org/ontology/biopharma/BiopharmaMaterial/
SubClass Of: mixed material
Class Hierarchy
owl:Thing › bfo:entity › bfo:continuant › bfo:independent continuant › bfo:material entity › mixed material › culture medium
Direct subclasses:
Definition
mixed material that is a liquid, semi-solid or solid formulation that provides essential nutrients (such as carbon, nitrogen, vitamins, and minerals) and controlled physicochemical conditions (such as pH and osmotic balance) required for the growth, maintenance, or specific metabolic activity of microorganisms or cells
Explanatory Notes
1) “Specific metabolic activity” refers to those metabolic processes that are intentionally supported or induced by the medium in a manufacturing or experimental context. This includes the synthesis and secretion of desired biomolecules (e.g., recombinant proteins, metabolites) or the replication and viability of cells when the cells themselves are the intended product. It excludes background or non-targeted metabolic processes such as basal energy maintenance or stress responses not relevant to the production goal. 2) In the context of biomanufacturing, “specific metabolic activity” typically refers to the set of biochemical processes carried out by microorganisms or cells that result in the synthesis, modification, and/or secretion of desired biological products. This includes processes such as the expression of recombinant proteins (e.g., monoclonal antibodies, enzymes), production of metabolites (e.g., amino acids, organic acids, antibiotics), or replication of the cells themselves when they are the product. These activities require the uptake and transformation of nutrients and are influenced by the composition and physicochemical properties of the culture medium. 3) Some types of culture media also include selection agents or antibiotics to suppress the growth of unwanted or contaminating organisms.
Examples
- DMEM (Dulbecco’s Modified Eagle’s Medium); LB (Lysogeny Broth) agar; AMBIC (Advanced Mammalian Biomanufacturing Innovation Center) medium
Adapted From
- https://pmc.ncbi.nlm.nih.gov/articles/PMC7417654/pdf/fbioe-08-00911.pdf and https://www.sigmaaldrich.com/US/en/technical-documents/technical-article/microbiological-testing/microbial-culture-media-preparation/types-of-media-in-microbiology?srsltid=AfmBOopAW7ApixpJ8J-96cmEfgfSiT8dX8wszs06BrclD3tYImJkEcYV
Primitive Class
This class is declared primitive and it does not have necessary and sufficient condiftions defined.
Primitive Rationale
This term is expected to remain primitive because it encompasses a broad set of media that are used for different purposes and have different compositions
Formal Axioms
First-Order Logic Axioms
CultureMedium(x) → MixedMaterial(x)
Semi-Formal Natural Language Axioms
if x is a ‘culture medium’ then x is a ‘mixed material’
Description Logic
constr:CultureMedium ⊑ constr:MixedMaterial
constr:CultureMedium ⊓ constr:Cell ⊑ ⊥
SubClassOf: constr:MixedMaterial
constr:CultureMedium DisjointWith: (constr:Cell, constr:CellCulture, constr:CulturedCellPopulation)