cell culture recovery process

IRI: https://spec.industrialontologies.org/ontology/construct/CellCultureRecoveryProcess

Defined In: https://spec.industrialontologies.org/ontology/biopharma/BiopharmaManufacturingExecution/

SubClass Of: planned process

Class Hierarchy

owl:Thing › bfo:entity › bfo:occurrent › bfo:process › planned process › cell culture recovery process

Definition

planned process in which cells that have undergone a stress-inducing process are cultured under controlled conditions to restore or stabilize viability, metabolic activity, or growth potential

Explanatory Notes

1) Recovery cultivation is applied when cells have experienced physiological stress that may compromise viability, metabolic activity, adaptation to culture conditions, or growth potential. Thawing from cryopreservation is a common example, because ice crystal formation, osmotic imbalance, and cryoprotectant exposure may require time for cells to stabilize after thaw. Similar recovery may be needed after other stress-inducing manipulations, such as shear exposure, nutrient deprivation, transfer between culture environments, or other handling stresses. 2) In post-thaw recovery cultivation, cells are often more vulnerable than during later expansion stages and may require careful handling under controlled conditions. Cells in post-thaw recovery are not necessarily in exponential growth; the focus is typically on stabilization, viability recovery, and adaptation to the culture environment. Acceptance criteria for advancing out of recovery may therefore differ from those used for exponential expansion, reflecting the physiological state of the cells during recovery. 3) Recovery cultivation should be distinguished from cell culture expansion process. Expansion is primarily directed toward increasing cell number through proliferation, whereas recovery cultivation is primarily directed toward restoring or stabilizing cell condition after stress. Some proliferation may occur during recovery, but proliferation is not the defining purpose of the process.

Examples

• Recovery of cryopreserved CHO Cells after thawing; Recovery of mammalian cells after transfer to serum-free medium

Adapted From

• https://documents.thermofisher.com/TFS-Assets/BID/Handbooks/gibco-cell-culture-basics-handbook.pdf and https://www.atcc.org/the-science/culturing-cells/reviving-cells#:~:text=The%20viability%20for%20most%20cells,recover%20and%20enter%20exponential%20growth. and https://www.thermofisher.com/rs/en/home/life-science/cloning/cloning-learning-center/invitrogen-school-of-molecular-biology/molecular-cloning/transformation/bacterial-transformation-workflow.html

Primitive Class

This class is declared primitive and it does not have necessary and sufficient condiftions defined.

Primitive Rationale

Insufficient constructs are present to create a set of necessary and sufficient conditions.

Formal Axioms

First-Order Logic Axioms

CellCultureRecoveryProcess(x) → PlannedProcess(x) ∧ ∃c (CellCulture(c) ∧ hasParticipantAtSomeTime(x, c))

Semi-Formal Natural Language Axioms

if x is a ‘cell culture recovery process’ then x is a ‘planned process’ that ‘has participant at some time’ some ‘cell culture’

Description Logic

constr:CellCultureRecoveryProcess ⊑ constr:PlannedProcess

constr:CellCultureRecoveryProcess ⊑ ∃ bfo:has_participant_at_some_time .constr:CellCulture

constr:CellCultureRecoveryProcess ⊓ constr:CellCultureExpansionProcess ⊑ ⊥

constr:CellCultureRecoveryProcess ⊓ constr:ProductionCultureProductionPhase ⊑ ⊥

SubClassOf: constr:PlannedProcess

SubClassOf: bfo:has_participant_at_some_time some constr:CellCulture

constr:CellCultureRecoveryProcess DisjointWith: (constr:CellCultureExpansionProcess, constr:ProductionCultureProductionPhase)