culture medium supplement role

IRI: https://spec.industrialontologies.org/ontology/construct/CultureMediumSupplementRole

Defined In: https://spec.industrialontologies.org/ontology/biopharma/BiopharmaMaterial/

SubClass Of: material component role

Class Hierarchy

owl:Thing › bfo:entity › bfo:continuant › bfo:specifically dependent continuant › bfo:realizable entity › bfo:role › material component role › culture medium supplement role

Definition

material component role held by a material entity when it is added or planned to be added to culture media to enrich or modify the medium composition or functional properties

Explanatory Notes

1) This role is context-dependent: a substance only counts as a media supplement when it is actually used or planned to be used to supplement culture media. The same chemical could serve other purposes in other settings (for example, insulin as a therapeutic drug). However, many commercial products (such as ITS mixes, lipid concentrates, or amino acid solutions) are specifically formulated and sold for use as media supplements. In practice, these preparations almost always bear a media supplement role. 2) Role may be held by single components and their solutions (such as insulin, transferrin, or glutamine) or prepared mixtures supplied as concentrated supplement solutions (e.g., ITS+3, MEM non-essential amino acids). 3) Media supplements are distinct from the medium itself (basal/complete formulations are not “supplements”). A component that is part of a basal recipe does not bear this role but a component added on top of a basal medium does. 4) Media supplements are separate from nutrient feeds and the additions during cultivation. When the purpose is to replenish depleted nutrients during cultivation the nutrient feed role should be used instead.

Examples

• Albumin stock solution bearing a media supplement role when added to serum-free medium to stabilize cells; ITS+3 Liquid Media Supplement (100×), a defined mixture of insulin, human transferrin, sodium selenite, linoleic acid, oleic acid bearing a media supplement role when added to serum-free medium to support viability and specialized functions;

Counter-Examples

• Albumin used as a blocking agent in immunoassays; Dulbecco’s Modified Eagle Medium (DMEM), a complete culture medium formulation providing baseline nutrients, is a culture medium and not a media supplement; Sterile water used as a solvent to reconstitute culture medium powder

Adapted From

• https://www.labome.com/method/Cell-Culture-Media-A-Review.html and https://www.thermofisher.com/rs/en/home/life-science/cell-culture/mammalian-cell-culture/media-supplements.html

Primitive Class

This class is declared primitive and it does not have necessary and sufficient condiftions defined.

Primitive Rationale

There are insufficient constructs to create necessary and sufficient conditions

Formal Axioms

First-Order Logic Axioms

CultureMediumSupplementRole(x) → MaterialComponentRole(x) ∧ ∃m (MaterialEntity(m) ∧ roleOf(x, m) ∧ ((∃c (CultureMedium(c) ∧ componentPartOfAtAllTimes(m, c))) ∨ ∃i∃p∃q (InputSpecification(i) ∧ prescribedBy(m, i) ∧ PlanSpecification(p) ∧ continuantPartOfAtAllTimes(i, p) ∧ prescribes(p, q) ∧ PlannedProcess(q) ∧ ∃c (CultureMedium(c) ∧ hasParticipantAtSomeTime(q, c)))))

Semi-Formal Natural Language Axioms

if x is a ‘culture medium supplement role’ then x is a ‘material component role’ that is the ‘role of’ some ‘material entity’ that either is a ‘component part of at all times’ some ‘culture medium’, or is ‘prescribed by’ some ‘input specification’ that is a ‘continuant part of at all times’ some ‘plan specification’ that ‘prescribes’ some ‘planned process’ that ‘has participant at some time’ some ‘culture medium’

Description Logic

constr:CultureMediumSupplementRole ⊑ constr:MaterialComponentRole

constr:CultureMediumSupplementRole ⊑ ∃ constr:roleOf .(bfo:material_entity ⊓ (∃ constr:componentPartOfAtAllTimes .constr:CultureMedium ⊔ ∃ constr:prescribedBy .(constr:InputSpecification ⊓ ∃ bfo:continuant_part_of_at_all_times .(constr:PlanSpecification ⊓ ∃ constr:prescribes .(constr:PlannedProcess ⊓ ∃ bfo:has_participant_at_some_time .constr:CultureMedium)))))

SubClassOf: constr:MaterialComponentRole

SubClassOf: constr:roleOf some (bfo:material_entity and (constr:componentPartOfAtAllTimes some constr:CultureMedium or constr:prescribedBy some (constr:InputSpecification and bfo:continuant_part_of_at_all_times some (constr:PlanSpecification and constr:prescribes some (constr:PlannedProcess and bfo:has_participant_at_some_time some constr:CultureMedium)))))