chelating agent role
IRI: https://spec.industrialontologies.org/ontology/construct/ChelatingAgentRole
Defined In: https://spec.industrialontologies.org/ontology/biopharma/MolecularEntity/
SubClass Of: molecular entity role
Class Hierarchy
owl:Thing › bfo:entity › bfo:continuant › bfo:specifically dependent continuant › bfo:realizable entity › bfo:role › molecular entity role › chelating agent role
Definition
role held by a molecular entity when it is used or planned to be used to bind a metal ion through two or more coordination sites
Explanatory Notes
1) The environment may be a solution, resin, gel, or other medium in which a metal ion can form coordination bonds. 2) Chelating agents are used for different purposes, such as preventing metal-catalyzed degradation, removing or masking trace metals, maintaining metal ion availability, or supporting analytical and purification steps where metal ions or metal ion bound biomolecules are captured, removed, or released.
Examples
- Role held by ethylenediaminetetraacetic acid (EDTA) acting as a chelating agent in buffers to remove trace metal ions; ethyleneglycol-bis(beta-aminoethylether)-N,N,N’,N’-tetraacetic acid (EGTA) bearing a selective calcium chelating agent role in calcium-sensitive assays; citric acid bearing a chelating agent role and regulating the presence of iron in culture media; nitrilotriacetate groups immobilized on an affinity resin and bearing a chelating agent role during metal-ion coordination
Counter-Examples
- Histidine added as a buffering component rather than for chelation; citrate added to adjust pH or provide a carbon source rather than to bind metals; magnesium ions acting as cofactors
Adapted From
- http://purl.obolibrary.org/obo/CHEBI_38161 and https://www.sigmaaldrich.com/RS/en/technical-documents/technical-article/protein-biology/protein-purification/chelators?srsltid=AfmBOooXIbg6UbKJhWf8NjXdHt19Ktk2E5gud8zwrHR5iuqvICV2jrN8 and https://www.merckmillipore.com/CH/en/products/chemistry-and-biochemicals/biochemicals/chelating-reducing-agents
Primitive Class
This class is declared primitive and it does not have necessary and sufficient condiftions defined.
Primitive Rationale
There are insufficient constructs to create a set of necessary and sufficient conditions
Formal Axioms
First-Order Logic Axioms
ChelatingAgentRole(x) → MolecularEntityRole(x)
Semi-Formal Natural Language Axioms
if x is a ‘chelating agent role’ then x is a ‘molecular entity role’