harvesting process

IRI: https://spec.industrialontologies.org/ontology/construct/HarvestingProcess

Defined In: https://spec.industrialontologies.org/ontology/biopharma/BiopharmaManufacturingExecution/

SubClass Of: purification process

Class Hierarchy

owl:Thing › bfo:entity › bfo:occurrent › bfo:process › planned process › manufacturing process › purification process › harvesting process

Definition

purification process that has as input the specified output of a biomanufacturing production process and that results in the separation or recovery of biotechnology products from the associated process streams, typically through the isolation or removal of cells, cell debris, or other bulk impurities

Explanatory Notes

Harvesting utilizes one or more sequential unit operations, such as centrifugation, filtration, cell concentration and membrane separation. The specific unit operations depend on whether the target product is located inside the cells (intracellular) or secreted into the medium (extracellular), or the cells themselves.

Examples

• Harvesting was carried out by depth filtration of a CHO cell culture to remove cells and debris, yielding a clarified supernatant containing the secreted monoclonal antibody for downstream purification; Harvesting was performed by collecting E. coli cells at early log phase (OD₆₀₀ ≈ 0.3–0.4) during cultivation at 37 °C. The culture was rapidly chilled on ice and centrifuged at 4 °C and 4,000 × g for 10 minutes to separate the biomass from the culture medium;

Adapted From

• https://books.google.rs/books?hl=sr&lr=&id=p-rKBQAAQBAJ&oi=fnd&pg=PP1&dq=bioseparation+processes+harvesting&ots=eFDtLSUJ0I&sig=XjzTFnAmknXNjFlSWHpkkTtpHg0&redir_esc=y#v=onepage&q=bioseparation%20processes%20harvesting&f=false

Primitive Class

This class is declared primitive and it does not have necessary and sufficient condiftions defined.

Primitive Rationale

Insufficient constructs are present to create a set of necessary and sufficient conditions

Formal Axioms

First-Order Logic Axioms

HarvestingProcess(x) → PurificationProcess(x) ∧ ∃m∃p(ProcessIntermediateMaterial(m) ∧ BiomanufacturingProductionProcess(p) ∧ isSpecifiedOutputOf(m, p) ∧ hasInput(x, m))

Semi-Formal Natural Language Axioms

if x is a ‘harvesting process’ then x is a ‘purification process’ that ‘has input’ some ‘process intermediate material’ which ‘is specified output of’ some ‘biomanufacturing production process’

Description Logic

constr:HarvestingProcess ⊑ constr:PurificationProcess

constr:HarvestingProcess ⊑ ∃ constr:hasInput .(constr:ProcessIntermediateMaterial ⊓ ∃ constr:isSpecifiedOutputOf .constr:BiomanufacturingProductionProcess)

SubClassOf: constr:PurificationProcess

SubClassOf: constr:hasInput some (constr:ProcessIntermediateMaterial and constr:isSpecifiedOutputOf some constr:BiomanufacturingProductionProcess)