complex galactosylated N-glycan moiety

IRI: https://spec.industrialontologies.org/ontology/construct/ComplexGalactosylatedNGlycanMoiety

Defined In: https://spec.industrialontologies.org/ontology/biopharma/MolecularEntity/

SubClass Of: biantennary complex N-glycan moiety

Class Hierarchy

owl:Thing › bfo:entity › bfo:continuant › bfo:independent continuant › bfo:material entity › chemical moiety › N-glycan moiety › core pentasaccharide N-glycan moiety › biantennary complex N-glycan moiety › complex galactosylated N-glycan moiety

Definition

biantennary complex N-glycan moiety that has as part one or more galactose residues

Semi-Formal Definition:

every instance of ‘complex galactosylated N-glycan moiety’ is defined as exactly an instance of ‘biantennary complex N-glycan moiety’ that ‘has proper continuant part at all times’ some ‘galactose residue’

Explanatory Notes

In biopharmaceutical analysis, ‘galactosylation’ commonly refers to the presence of galactose residues on the antennae of complex biantennary N-glycans. Complex biantennary glycans have two N-acetylglucosamine–initiated antennae that can receive galactose, and galactosylation levels are routinely reported through the relative abundance of mono-galactosylated (G1) and di-galactosylated (G2) complex species. Hybrid glycans may carry a galactose residue on a GlcNAc-extended arm, but they are not typically categorized as G1/G2 complex species and are usually not included in percent galactosylation calculations for complex Fc glycan profiles. High-mannose glycans do not contain complex-type antennae and are not treated as galactosylated complex N-glycans. Accordingly, this class applies to complex N-glycan moieties that contain one or more galactose residues

Examples

G1F biantennary complex N-glycan moiety with one galactose residue; G2F biantennary complex N-glycan moiety with two galactose residues; G1 biantennary complex N-glycan moiety with one galactose residue and no fucose residue; Galactosylated Fc N-glycan moiety detected during monoclonal antibody glycan profiling

Adapted From

https://www.ema.europa.eu/en/documents/scientific-guideline/guideline-development-production-characterisation-and-specification-monoclonal-antibodies-and-related-products-revision-1_en.pdf and http://www.jbiomed.com/v04p0035.htm and https://pmc.ncbi.nlm.nih.gov/articles/PMC4634315/

Formal Axioms

First-Order Logic Definition

ComplexGalactosylatedNGlycanMoiety(x) ↔ BiantennaryComplexNGlycanMoiety(x) ∧ ∃y (hasProperContinuantPartAtAllTimes(x, y) ∧ GalactoseResidue(y))

Description Logic

constr:ComplexGalactosylatedNGlycanMoiety ≡ constr:BiantennaryComplexNGlycanMoiety ⊓ ∃ bfo:has_proper_continuant_part_at_all_times .constr:GalactoseResidue

constr:ComplexGalactosylatedNGlycanMoiety ⊑ constr:BiantennaryComplexNGlycanMoiety

constr:ComplexGalactosylatedNGlycanMoiety ⊓ constr:ComplexAGalactosylatedNGlycanMoiety ⊑ ⊥

EquivalentTo: constr:BiantennaryComplexNGlycanMoiety and bfo:has_proper_continuant_part_at_all_times some constr:GalactoseResidue

SubClassOf: constr:BiantennaryComplexNGlycanMoiety

constr:ComplexGalactosylatedNGlycanMoiety DisjointWith: constr:ComplexAGalactosylatedNGlycanMoiety