reverse phase chromatography process
IRI: https://spec.industrialontologies.org/ontology/construct/ReversePhaseChromatographyProcess
Defined In: https://spec.industrialontologies.org/ontology/biopharma/BiopharmaManufacturingExecution/
SubClass Of: liquid chromatography process
Class Hierarchy
owl:Thing › bfo:entity › bfo:occurrent › bfo:process › planned process › chromatography process › liquid chromatography process › reverse phase chromatography process
Definition
liquid chromatography process in which the stationary phase is nonpolar and the mobile phase is polar, resulting in the separation of components based on their relative hydrophobicity
Explanatory Notes
Reverse-phase chromatography (RPC) is utilized for the purification, separation, and analysis of biological molecules, including proteins, peptides, antibodies, and other metabolites. It is also employed in the separation and purification of intermediates and final products in chemical synthesis processes.
Examples
- Chromatography process in which a synthetic peptide was purified to remove synthesis byproducts using a C18-bonded silica stationary phase and a mobile phase composed of water and acetonitrile with 0.1% trifluoroacetic acid (TFA); Chromatography process in which a monoclonal antibody digest was resolved to quantify fragment abundance using a hydrophobic C18 stationary phase and a mobile phase gradient of water and methanol with 0.1% formic acid
Adapted From
- http://wolfson.huji.ac.il/purification/PDF/affinity/GE_Affinity_Chromatography_Handbook-Antibodies.pdf and http://purl.obolibrary.org/obo/CHMO_0002302 and https://www.sigmaaldrich.com/RS/en/technical-documents/protocol/analytical-chemistry/purification/reverse-phased-chromatography-in-practice?srsltid=AfmBOorOQim54doE5guL_YoH41EUR9xT6oikpa5rJhbNFAYeFTiIxs0I
Primitive Class
This class is declared primitive and it does not have necessary and sufficient condiftions defined.
Primitive Rationale
Insufficient constructs are present to create a set of necessary and sufficient conditions
Formal Axioms
First-Order Logic Axioms
ReversePhaseChromatographyProcess(x) → LiquidChromatographyProcess(x)
Semi-Formal Natural Language Axioms
if x is a ‘reverse phase chromatography process’ then x is a ‘liquid chromatography process’