chemically defined culture medium
IRI: https://spec.industrialontologies.org/ontology/construct/ChemicallyDefinedCultureMedium
Defined In: https://spec.industrialontologies.org/ontology/biopharma/BiopharmaMaterial/
SubClass Of: serum free culture medium
Class Hierarchy
owl:Thing › bfo:entity › bfo:continuant › bfo:independent continuant › bfo:material entity › mixed material › culture medium › serum free culture medium › chemically defined culture medium
Definition
serum free culture medium in which each component and its concentration are known and reproducible, and that excludes undefined components such as serum, extracts, or hydrolysates
Explanatory Notes
Chemically defined media (CDM) are culture media composed only of pure, quantified ingredients of known chemical identity and concentration. They exclude animal-derived serum and complex additives such as yeastolate, peptones, or hydrolysates. Recombinant proteins (e.g., insulin, transferrin) may be included provided their identity and concentration are specified. CDMs improve control, reproducibility, and lot-to-lot consistency, simplify downstream purification, and enhance comparability between experiments or production runs. In biopharmaceutical manufacturing, they are favored for reducing regulatory concerns associated with serum and undefined additives.
Examples
- ExpiCHO Expression Medium is chemically defined, serum-free, used in high-density fed-batch processes
Counter-Examples
- Media with plasma-derived transferrin (without purity specification) is not strictly chemically defined unless concentration is characterized
Adapted From
- https://www.labome.com/method/Cell-Culture-Media-A-Review.html
Primitive Class
This class is declared primitive and it does not have necessary and sufficient condiftions defined.
Primitive Rationale
There are insufficient constructs available to create a set of necessary and sufficient conditions.
Formal Axioms
First-Order Logic Axioms
ChemicallyDefinedCultureMedium(x) → SerumFreeCultureMedium(x)
Semi-Formal Natural Language Axioms
if x is a ‘chemically defined culture medium’ then x is a ‘serum free culture medium’