spent culture media analysis
IRI: https://spec.industrialontologies.org/ontology/construct/SpentCultureMediaAnalysis
Defined In: https://spec.industrialontologies.org/ontology/biopharma/BiopharmaMaterialTesting/
SubClass Of: measurement process
Class Hierarchy
owl:Thing › bfo:entity › bfo:occurrent › bfo:process › planned process › measurement process › spent culture media analysis
Definition
measurement process in which one or more attributes of material samples containing spent culture medium are measured to characterize the extracellular medium during or following cell culture
Explanatory Notes
1) Spent culture media analysis may use samples taken from culture medium directly or from a cell culture that contains culture medium in a spent culture medium state. In many workflows, the sampled material may initially contain cells, particulates, or other culture-associated material before clarification, centrifugation, filtration, or other sample preparation. 2) Results from spent culture media analysis may be compared with measurements from fresh medium or with measurements obtained at earlier sampling points to evaluate nutrient depletion, metabolite accumulation, or other changes during cell culture.
Examples
- measurement of glucose, glutamine, lactate, and ammonia concentrations in clarified spent-culture medium samples collected from a CHO cell culture at specified process times; targeted measurement of amino-acid concentrations in spent-culture medium samples collected before and after a culture-feed addition; LC-MS profiling of extracellular metabolites in spent-culture medium samples collected from reference and perturbed cell-culture runs
Adapted From
- https://api.sartorius.com/document-hub/dam/download/247594/Spent-Media-Analytics-whitepaper-en-B.pdf
Primitive Class
This class is declared primitive and it does not have necessary and sufficient condiftions defined.
Primitive Rationale
There are insufficient constructs to create a set of necessary and sufficient conditions.
Formal Axioms
First-Order Logic Axioms
SpentCultureMediaAnalysis(x) → MeasurementProcess(x) ∧ ∃s (MaterialSample(s) ∧ hasInput(x, s) ∧ ∃p (MaterialSamplingProcess(p) ∧ isSpecifiedOutputOf(s, p) ∧ ∃q(hasPreMaterialState(p, q) ∧ SpentCultureMediumState(q)))))
Semi-Formal Natural Language Axioms
if x is a ‘spent culture media analysis’ then x is a ‘measurement process’ and x ‘has input’ some ‘material sample’ that ‘is specified output of’ some ‘material sampling process’ that ‘has pre material state’ some ‘spent culture medium state’
Description Logic
constr:SpentCultureMediaAnalysis ⊑ constr:MeasurementProcess
constr:SpentCultureMediaAnalysis ⊑ ∃ constr:hasInput .(constr:MaterialSample ⊓ ∃ constr:isSpecifiedOutputOf .(constr:MaterialSamplingProcess ⊓ ∃ constr:hasPreMaterialState .constr:SpentCultureMediumState))
SubClassOf: constr:MeasurementProcess
SubClassOf: constr:hasInput some (constr:MaterialSample and constr:isSpecifiedOutputOf some (constr:MaterialSamplingProcess and constr:hasPreMaterialState some constr:SpentCultureMediumState))