spent culture medium state
IRI: https://spec.industrialontologies.org/ontology/construct/SpentCultureMediumState
Defined In: https://spec.industrialontologies.org/ontology/biopharma/BiopharmaMaterial/
SubClass Of: material state
Class Hierarchy
owl:Thing › bfo:entity › bfo:occurrent › bfo:process › material state › spent culture medium state
Definition
material state of culture medium that has participated in a cell cultivation process and is characterized by compositional changes caused by cellular activity, process additions, or accumulation of extracellular material
Explanatory Notes
A spent culture medium state is a state of culture medium during or after cultivation. It may involve depletion of nutrients, accumulation of metabolites such as lactate or ammonia, secretion of product or host cell proteins, changes in pH or osmolality, or other extracellular compositional changes. The term does not imply that the medium is fully exhausted or unsuitable for continued cultivation.
Examples
- State of culture medium in a CHO fed-batch culture after several days of glucose and amino acid consumption; State of culture medium containing accumulated lactate and ammonia during mammalian cell cultivation; State of culture medium containing secreted recombinant protein and host cell proteins at harvest; State of culture medium after feed addition and cellular metabolism have changed its nutrient and metabolite profile; State of culture medium sampled from a bioreactor during cultivation for extracellular metabolite analysis; State of culture medium remaining after cultivation before clarification or downstream processing
Primitive Class
This class is declared primitive and it does not have necessary and sufficient condiftions defined.
Primitive Rationale
There are insufficient constructs for creating a set of necessary and sufficient conditions
Formal Axioms
First-Order Logic Axioms
SpentCultureMediumState(x) → MaterialState(x) ∧ ∃m (CultureMedium(m) ∧ isMaterialStateOf(x, m))
Semi-Formal Natural Language Axioms
if x is a ‘spent culture medium state’ then x is a ‘material state’ that ‘is material state of’ some ‘culture medium’