cell culture expansion process
IRI: https://spec.industrialontologies.org/ontology/construct/CellCultureExpansionProcess
Defined In: https://spec.industrialontologies.org/ontology/biopharma/BiopharmaManufacturingExecution/
SubClass Of: planned process
Class Hierarchy
owl:Thing › bfo:entity › bfo:occurrent › bfo:process › planned process › cell culture expansion process
Direct subclasses:
Definition
planned cell culture process during which cultivation is managed primarily to increase the total number of cells through cell proliferation
Explanatory Notes
Implicit in cell culture expansion is the assumption that cells in culture remain healthy and proliferative. This assumption is typically evaluated by monitoring cell viability, which serves as a key indicator of culture fitness.
Incidental or secondary cell proliferation during a cell culture recovery process or production culture production phase does not by itself make that process a cell culture expansion process.
Examples
- Shake flask expansion of CHO cells with controlled feeding and monitoring; seed train expansion of hematopoietic stem cells under GMP conditions; fed-batch bioreactor expansion of hybridoma cells with automated pH and DO control; scale-up culture of mammalian cells in rocking bioreactors with defined agitation parameters; T cell expansion in gas-permeable bags with scheduled media additions
Counter-Examples
- proliferation of cells caused by a contamination
Adapted From
- OBI:0001147
Primitive Class
This class is declared primitive and it does not have necessary and sufficient condiftions defined.
Primitive Rationale
Insufficient constructs are present to create a set of necessary and sufficient conditions.
Formal Axioms
First-Order Logic Axioms
CellCultureExpansionProcess(x) → PlannedProcess(x) ∧ ∃p (CellPopulationProliferation(p) ∧ hasOccurrentPart(x, p)) ∧ ∃c (CellCulture(c) ∧ hasParticipantAtSomeTime(x, c))
Semi-Formal Natural Language Axioms
if x is a ‘cell culture expansion process’ then x is a ‘planned process’ that ‘has occurrent part’ some ‘cell population proliferation’ and ‘has participant at some time’ some ‘cell culture’
Description Logic
constr:CellCultureExpansionProcess ⊑ constr:PlannedProcess
constr:CellCultureExpansionProcess ⊑ ∃ bfo:has_participant_at_some_time .constr:CellCulture
constr:CellCultureExpansionProcess ⊑ ∃ bfo:has_occurrent_part .constr:CellPopulationProliferation
constr:CellCultureExpansionProcess ⊓ constr:CellCultureRecoveryProcess ⊑ ⊥
constr:CellCultureExpansionProcess ⊓ constr:ProductionCultureProductionPhase ⊑ ⊥
SubClassOf: constr:PlannedProcess
SubClassOf: bfo:has_participant_at_some_time some constr:CellCulture
SubClassOf: bfo:has_occurrent_part some constr:CellPopulationProliferation
constr:CellCultureExpansionProcess DisjointWith: (constr:CellCultureRecoveryProcess, constr:ProductionCultureProductionPhase)